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ms2 p65 hsf1 gfp vector  (Addgene inc)


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    Addgene inc ms2 p65 hsf1 gfp vector
    Ms2 P65 Hsf1 Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+gfp/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/bio_rxiv__64898__2026__03__01__708923-177-6-13
    Average 94 stars, based on 82 article reviews
    ms2 p65 hsf1 gfp vector - by Bioz Stars, 2026-10
    94/100 stars

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    Transfection:

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [ ]. ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing speci c sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) (Konermann et al. 2015). ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST.
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [45]. ..

    Plasmid Preparation:

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [ ]. ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing speci c sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) (Konermann et al. 2015). ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST.
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [45]. ..

    Article Title: Supporting Information Chemical control of a CRISPR-Cas9 acetyltransferase
    Article Snippet: General procedures6: pcDNA-dCas9-VP64 (Addgene plasmid # 47107), pcDNA-dCas9-HA (Addgene plasmid # 61355), pcDNA-dCas9-p300 Core (Addgene plasmid # 61357), and pcDNA-dCas9-p300 Core (D1399Y) (Addgene plasmid # 61358) were a gift from Charles Gersbach. .. MS2-P65-HSF1_GFP was a gift from Feng Zhang (Addgene plasmid # 61423). .. SuperBroth including the appropriate antibiotic was inoculated with bacteria from Addgene agar stabs for plasmids 47107, 61355, 61357, 61358, and 61423 and grown overnight at 30 degrees Celsius in a shaking incubator.

    Article Title: NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndrome.
    Article Snippet: .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA). ..

    Article Title: NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndrome
    Article Snippet: .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA). .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA).

    Incubation:

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [ ]. ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing speci c sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) (Konermann et al. 2015). ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST.
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [45]. ..

    Cloning:

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [ ]. ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing speci c sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) (Konermann et al. 2015). ..

    Article Title: Genetic analysis of cis-enhancers associated with bone mineral density and periodontitis in the gene SOST.
    Article Snippet: .. For CRISPRa, each 6-well was transfected with 1 μg sgRNAs(MS2) (containing specific sgRNA(s)), 1 μg dCAS9-VP64_GFP [plasmid #61422], and 1 μg MS2-P65-HSF1_GFP [plasmid #61423] and incubated for 44 h. All plasmids were obtained from Addgene (cloning backbone plasmids was a gift from Feng Zhang) [45]. ..

    Article Title: NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndrome.
    Article Snippet: .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA). ..

    Article Title: NEAT1-mediated regulation of proteostasis and mRNA localization impacts autophagy dysregulation in Rett syndrome
    Article Snippet: .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA). .. For this approach, we used the following three plasmids: single-guide RNA (sgRNA) [MS2; a guide cloning backbone (Plasmid #61424)], dCAS9-VP64_GFP (Plasmid #61422), and MS2-P65-HSF1_GFP (Plasmid #61423), which were acquired from Addgene (Watertown, MA, USA).

    Activation Assay:

    Article Title: Defective proviruses cause T cell reprogramming through promoter exaptation in HIV-1 infection
    Article Snippet: .. In brief, 5*10 5 cells were seeded in a six-well plate and co-transfected with 1.4 μg sgRNA(MS2)-gRNA for either LTR or BACH2 activation, 0,8 μg dCas9-VP64_GFP (Addgene #61422) and 1 μg MS2-P65-HSF1_GFP (Addgene #61423) per well. .. Transfection was performed using TransIT LT1 reagent (Mirus Bio) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Three-component CRISPR/Cas complex system and uses thereof
    Article Snippet: .. A fusion PCR of 5′ fragment of NLSPUFa with 3′ fragment of NLSPUb was used to create pAC1358:pmax-NLSPUFc_VP64. p65HSF1 activator ORF was amplified from MS2-P65-HSF1_GFP (Addgene: 61423) with FseI PacI sites to replace VP64 fragment in pAC164 to create pAC1410:pmax-dCas9_p65HSF1, and replace VP64 in pAC1355 and pAC1358 to create pAC1393: pmax-NLSPUFa_p65HSF1 and pAC1411:pmax-NLSPUFc_p65HSF1, respectively. .. Clover and mRuby2 were amplified from pcDNA3-Clover (Addgene #40259) and pcDNA3-mRuby2 (Addgene #40260) respectively with primers containing SgrAI and FseI cloning site, ligated with various FseI-PUF-PacI amplified from the above pAC1356 ̃1358 and vector digested from pAC149:pCR8-dCas9VP160 (Addgene #48221) to create gateway donor vectors pAC1402, pAC1403 and pAC1404 containing ORFs of Clover_PUFa and Clover_PUFc, mRuby2_PUFa, respectively.

    Amplification:

    Article Title: Three-component CRISPR/Cas complex system and uses thereof
    Article Snippet: .. A fusion PCR of 5′ fragment of NLSPUFa with 3′ fragment of NLSPUb was used to create pAC1358:pmax-NLSPUFc_VP64. p65HSF1 activator ORF was amplified from MS2-P65-HSF1_GFP (Addgene: 61423) with FseI PacI sites to replace VP64 fragment in pAC164 to create pAC1410:pmax-dCas9_p65HSF1, and replace VP64 in pAC1355 and pAC1358 to create pAC1393: pmax-NLSPUFa_p65HSF1 and pAC1411:pmax-NLSPUFc_p65HSF1, respectively. .. Clover and mRuby2 were amplified from pcDNA3-Clover (Addgene #40259) and pcDNA3-mRuby2 (Addgene #40260) respectively with primers containing SgrAI and FseI cloning site, ligated with various FseI-PUF-PacI amplified from the above pAC1356 ̃1358 and vector digested from pAC149:pCR8-dCas9VP160 (Addgene #48221) to create gateway donor vectors pAC1402, pAC1403 and pAC1404 containing ORFs of Clover_PUFa and Clover_PUFc, mRuby2_PUFa, respectively.



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